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Thetva (A) gene suspected to confer resistance to pleuromutilins inBrachyspira hyodysenteriae was tested for functionality inEscherichia coli AG100A andStaphylococcus aureus RN4220. Expression of the clonedtva (A) gene conferred decreased susceptibility to pleuromutilin (P) and streptogramin A (SA ) antibiotics inE. coli and had a minor effect inS. aureus . The finding provides evidence of the direct association oftva (A) with the PSA resistance phenotype.

The tva (A) Gene from Brachyspira hyodysenteriae Confers Decreased Susceptibility to Pleuromutilins and Streptogramin A in Escherichia coli