Open AccessNew Species Genetic Approach To Identify Strains of Mitis Group Streptococci That Are Donors of Rifampin Resistance to Streptococcus pneumoniae
Author(s) -
Marı́a José Ferrándiz,
Carmen Ardanuy,
Josefina Liñares,
Luz Balsalobre,
Marı́a Teresa Garcı́a,
Adela G. de la Campa
Publication year - 2011
Publication title -
antimicrobial agents and chemotherapy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.07
H-Index - 259
eISSN - 1070-6283
pISSN - 0066-4804
DOI - 10.1128/aac.00856-10
Subject(s) - rpob , streptococcus mitis , microbiology and biotechnology , biology , streptococcus pneumoniae , streptococcus oralis , streptococcaceae , viridans streptococci , streptococcus , 16s ribosomal rna , antibiotics , genetics , gene , bacteria
Eight rifampin-resistant streptococci of the mitis group were identified at the species level by using a concatenated 16S rRNA gene-sodA-rpoB-hlpA sequence. Characterization of theirrpoB alleles showed single amino acid changes involved in rifampin resistance. Comparison of RpoB sequences from pneumococcal recombinant isolates, viridans isolates, and type strains revealed a species-specific amino acid signature, which allowed it to be ascertained that recombinant RpoBs were originated in genetic interchanges withStreptococcus mitis andStreptococcus oralis .