Synergistic Efficacy of Aedes aegypti Antimicrobial Peptide Cecropin A2 and Tetracycline against Pseudomonas aeruginosa

The increasing prevalence of antibiotic resistance has created an urgent need for alternative drugs with new mechanisms of action. Antimicrobial peptides (AMPs) are promising candidates that could address the spread of multidrug-resistant bacteria, either alone or in combination with conventional antibiotics. We studied the antimicrobial efficacy and bactericidal mechanism of cecropin A2, a 36-residue α-helical cationic peptide derived fromAedes aegypti cecropin A, focusing on the common pathogenPseudomonas aeruginosa . The peptide showed little hemolytic activity and toxicity toward mammalian cells, and the MICs against most clinicalP. aeruginosa isolates were 32 to 64 μg/ml, and its MICs versus other Gram-negative bacteria were 2 to 32 μg/ml. Importantly, cecropin A2 demonstrated synergistic activity againstP. aeruginosa when combined with tetracycline, reducing the MICs of both agents by 8-fold. The combination was also effectivein vivo in theP. aeruginosa/Galleria mellonella model (P < 0.001). We found that cecropin A2 bound toP. aeruginosa lipopolysaccharides, permeabilized the membrane, and interacted with the bacterial genomic DNA, thus facilitating the translocation of tetracycline into the cytoplasm. In summary, the combination of cecropin A2 and tetracycline demonstrated synergistic antibacterial activity againstP. aeruginosa in vitro andin vivo , offering an alternative approach for the treatment ofP. aeruginosa infections.