Characterization of Ribosomal Binding and Antibacterial Activities Using Two Orthogonal High-Throughput Screens | Zendy

Ada King | Zendy; Derrick Watkins | Zendy; Sunil Kumar | Zendy; Nihar Ranjan | Zendy; Changjun Gong | Zendy; Jarred M. Whitlock | Zendy; Dev P. Arya | Zendy

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Characterization of Ribosomal Binding and Antibacterial Activities Using Two Orthogonal High-Throughput Screens

Author(s) -

Ada King,

Derrick Watkins,

Sunil Kumar,

Nihar Ranjan,

Changjun Gong,

Jarred M. Whitlock,

Dev P. Arya

Publication year - 2013

Publication title -

antimicrobial agents and chemotherapy

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.07

H-Index - 259

eISSN - 1070-6283

pISSN - 0066-4804

DOI - 10.1128/aac.00671-13

Subject(s) - antibacterial activity , neomycin , ribosomal rna , ribosomal protein , chemistry , biology , biochemistry , rna , bacteria , antibiotics , ribosome , genetics , gene

We report here the affinity and antibacterial activity of a structurally similar class of neomycin dimers. The affinity of the dimer library for rRNA was established by using a screen that measures the displacement of fluorescein-neomycin (F-neo) probe from RNA. A rapid growth inhibition assay using a single drug concentration was used to examine the antibacterial activity. The structure-activity relationship data were then rapidly analyzed using a two-dimensional ribosomal binding-bacterial inhibition plot analysis.

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