Discrimination between Native and Tn 6010 -Associated oqxAB in Klebsiella spp., Raoultella spp., and other Enterobacteriaceae by Using a Two-Step Strategy | Zendy

Thomas Guillard | Zendy; Anne-Laure Lebreil | Zendy; Lars Hestbjerg Hansen | Zendy; Aymric Kisserli | Zendy; Sibel Berger | Zendy; Alain Lozniewski | Zendy; Corentine Alauzet | Zendy; Christophe de Champs | Zendy

Open Access

Discrimination between Native and Tn 6010 -Associated oqxAB in Klebsiella spp., Raoultella spp., and other Enterobacteriaceae by Using a Two-Step Strategy

Author(s) -

Thomas Guillard,

Anne-Laure Lebreil,

Lars Hestbjerg Hansen,

Aymric Kisserli,

Sibel Berger,

Alain Lozniewski,

Corentine Alauzet,

Christophe de Champs

Publication year - 2015

Publication title -

antimicrobial agents and chemotherapy

Language(s) - English

Resource type - Journals

eISSN - 1070-6283

pISSN - 0066-4804

DOI - 10.1128/aac.00669-15

Subject(s) - enterobacteriaceae , microbiology and biotechnology , biology , klebsiella , gene , medicine , genetics , escherichia coli

We developed a two-step PCR-based strategy to detect genes encoding OqxAB, allowing a specific assignment of Tn6010-associated oqxAB in Enterobacteriaceae. Chromosomal location in this setup was confirmed by hybridization with I-CeuI-restricted genomes. This approach led us to find that Klebsiella sp. and Raoultella sp. reference strains chromosomally carried oqxAB.

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