Recommendations To Address the Difficulties Encountered When Determining Linezolid Resistance from Whole-Genome Sequencing Data | Zendy

Alicia G. Beukers | Zendy; Henrik Hasman | Zendy; Kristin Hegstad | Zendy; Sebastiaan J. van Hal | Zendy

Open Access

Recommendations To Address the Difficulties Encountered When Determining Linezolid Resistance from Whole-Genome Sequencing Data

Author(s) -

Alicia G. Beukers,

Henrik Hasman,

Kristin Hegstad,

Sebastiaan J. van Hal

Publication year - 2018

Publication title -

antimicrobial agents and chemotherapy

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.07

H-Index - 259

eISSN - 1070-6283

pISSN - 0066-4804

DOI - 10.1128/aac.00613-18

Subject(s) - 23s ribosomal rna , numbering , genetics , biology , whole genome sequencing , mutation , ribosomal rna , confusion , genome , computational biology , cancer genome sequencing , gene , rna , computer science , algorithm , ribosome , psychology , psychoanalysis

Mutations associated with linezolid resistance within the V domain of 23S rRNA are annotated using anEscherichia coli numbering system. The 23S rRNA gene varies in length, nucleotide sequence, and copy number among bacterial species. Consequently, this numbering system is not intuitive and can lead to confusion when mutation sites are being located using whole-genome sequencing data. Using the mutation G2576T as an example, we demonstrate the difficulties associated with using theE. coli numbering system.

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