Open AccessLmrS Is a Multidrug Efflux Pump of the Major Facilitator Superfamily from Staphylococcus aureus
Author(s) -
Jody L. Floyd,
Kenneth P. Smith,
Sanath Kumar,
Jared T. Floyd,
Manuel F. Varela
Publication year - 2010
Publication title -
antimicrobial agents and chemotherapy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.07
H-Index - 259
eISSN - 1070-6283
pISSN - 0066-4804
DOI - 10.1128/aac.00580-10
Subject(s) - major facilitator superfamily , efflux , lincomycin , microbiology and biotechnology , staphylococcus aureus , linezolid , multiple drug resistance , chloramphenicol , biology , antibiotic resistance , antibiotics , bacteria , transporter , biochemistry , genetics , vancomycin , gene
A multidrug efflux pump designated LmrS (lincomycin resistance protein ofStaphylococcus aureus ), belonging to the major facilitator superfamily (MFS) of transporters, was cloned, and the role of LmrS in antimicrobial efflux was evaluated. The highest relative increase in MIC, 16-fold, was observed for linezolid and tetraphenylphosphonium chloride (TPCL), followed by an 8-fold increase for sodium dodecyl sulfate (SDS), trimethoprim, and chloramphenicol. LmrS has 14 predicted membrane-spanning domains and is homologous to putative lincomycin resistance proteins ofBacillus spp.,Lactobacillus spp., andListeria spp.