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We directly tested 484 organisms from clinical (n  = 310) and simulated (n  = 174) positive blood cultures using the NG-Test Carba 5 assay for carbapenemase-producingEnterobacterales detection. The assay identified all but 4 of the KPC (170/171), OXA-48-like (22/22), VIM (19/21), and NDM (14/15) producers with no false positives.

Simplified Testing Method for Direct Detection of Carbapenemase-Producing Organisms from Positive Blood Cultures Using the NG-Test Carba 5 Assay