Open AccessDevelopment of a Novel Formulation with Hypericin To Treat Cutaneous Leishmaniasis Based on Photodynamic Therapy inIn VitroandIn VivoStudies
Author(s) -
Andrés Montoya,
Alejandro Daza,
Diana L. Muñoz,
K. Rios,
Viviana M. Taylor,
David L. Cedeño,
Iván Darío Vélez,
Fernando Echeverri,
Sara M. Robledo
Publication year - 2015
Publication title -
antimicrobial agents and chemotherapy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.07
H-Index - 259
eISSN - 1070-6283
pISSN - 0066-4804
DOI - 10.1128/aac.00545-15
Subject(s) - hypericin , in vivo , hypericum perforatum , photodynamic therapy , in vitro , cutaneous leishmaniasis , pharmacology , photosensitizer , hamster , amastigote , phototoxicity , leishmania , biology , chemistry , leishmaniasis , microbiology and biotechnology , immunology , biochemistry , organic chemistry , parasite hosting , world wide web , computer science
An evaluation of the leishmanicidal activityin vitro andin vivo of hypericin, an expanded-spectrum photosensitizer found inHypericum perforatum , is presented. Hypericin was evaluated against intracellular amastigotesin vitro ofLeishmania (Viannia )panamensis . A topical formulation containing 0.5% hypericin was developed and assayedin vivo in a hamster model of cutaneous leishmaniasis. Results demonstrate that hypericin induces a significant antiamastigote effectin vitro againstL. panamensis by decreasing the number of parasites inside infected cells. The topical formulation of 0.5% hypericin allows healing ofL. panamensis -induced lesions upon a topical application of 40 mg/day plus visible-light irradiation (5 J/cm2 , 15 min), twice a week for 3 weeks.
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