Open AccessRapid and Sensitive Liquid Chromatography/Mass Spectrometry Assay for Caspofungin in Human Aqueous Humor
Author(s) -
Chin Fen Neoh,
Hui He,
Jian Li,
Robert Fullinfaw,
Lok Leung,
Anant Misra,
Rasik B Vajpayee,
Geoff Davies,
Kay Stewart,
David Chee Ming Kong
Publication year - 2010
Publication title -
antimicrobial agents and chemotherapy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.07
H-Index - 259
eISSN - 1070-6283
pISSN - 0066-4804
DOI - 10.1128/aac.00509-10
Subject(s) - chromatography , chemistry , mass spectrometry , liquid chromatography–mass spectrometry , aqueous solution , detection limit , acetonitrile , caspofungin , high performance liquid chromatography , medicine , antifungal , amphotericin b , dermatology
A rapid, precise, and sensitive liquid chromatography/mass spectrometry (LC/MS) method to quantify the caspofungin concentration in human aqueous humor was developed and validated. Sample preparation involved simple dilution of aqueous humor samples with acetonitrile. Azithromycin was the internal standard. Good linearity over 10 to 5,000 ng/ml was observed. The lower limit of quantification was 10 ng/ml. The intra- and interday accuracies (percent bias) were within 11%, while the intra- and interday precisions were within 6%.