Open AccessThe ABC Transporter AnrAB Contributes to the Innate Resistance of Listeria monocytogenes to Nisin, Bacitracin, and Various β-Lactam Antibiotics
Author(s) -
Barry Collins,
Nicola J. Curtis,
Paul D. Cotter,
Colin Hill,
R. Paul Ross
Publication year - 2010
Publication title -
antimicrobial agents and chemotherapy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.07
H-Index - 259
eISSN - 1070-6283
pISSN - 0066-4804
DOI - 10.1128/aac.00503-10
Subject(s) - nisin , bacitracin , microbiology and biotechnology , lantibiotics , listeria monocytogenes , biology , transposable element , efflux , atp binding cassette transporter , antibiotics , antibiotic resistance , lipid ii , mutant , bacteria , genetics , antimicrobial , gene , transporter , bacterial cell structure
Amariner transposon bank was used to identify loci that contribute to the innate resistance ofListeria monocytogenes to the lantibiotic nisin. In addition to highlighting the importance of a number of loci previously associated with nisin resistance (mprF ,virRS , andtelA ), a nisin-sensitive phenotype was associated with the disruption ofanrB (lmo2115 ), a gene encoding the permease component of an ABC transporter. The contribution ofanrB to nisin resistance was confirmed by the creation of nonpolar deletion mutants. The loss of this putative multidrug resistance transporter also greatly enhanced sensitivity to bacitracin, gallidermin, and a selection of β-lactam antibiotics. A comparison of the relative antimicrobial sensitivities of a number of mutants established the ΔanrB strain as being one of the most bacitracin-sensitiveL. monocytogenes strains identified to date.