A Model for Evaluating Topical Antimicrobial Efficacy against Methicillin-Resistant Staphylococcus aureus Biofilms in Superficial Murine Wounds | Zendy

Eric Roche | Zendy; Paul J. Renick | Zendy; Shan P. Tetens | Zendy; Dennis Carson | Zendy

Open Access

A Model for Evaluating Topical Antimicrobial Efficacy against Methicillin-Resistant Staphylococcus aureus Biofilms in Superficial Murine Wounds

Author(s) -

Eric Roche,

Paul J. Renick,

Shan P. Tetens,

Dennis Carson

Publication year - 2012

Publication title -

antimicrobial agents and chemotherapy

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.07

H-Index - 259

eISSN - 1070-6283

pISSN - 0066-4804

DOI - 10.1128/aac.00467-12

Subject(s) - staphylococcus aureus , biofilm , antimicrobial , microbiology and biotechnology , micrococcaceae , methicillin resistant staphylococcus aureus , in vivo , inoculation , medicine , staphylococcal infections , antibacterial agent , antibiotics , biology , bacteria , immunology , genetics

A wound biofilm model was created by adapting a superficial infection model. Partial-thickness murine wounds were inoculated with methicillin-resistantStaphylococcus aureus (MRSA). Dense biofilm communities developed at the wound surface after 24 h as demonstrated by microscopy and quantitative microbiology. Common topical antimicrobial agents had reduced efficacy when treatment was initiated 24 h after inoculation compared to 4 h after inoculation. This model provides a rapidin vivo test for new agents to treat wound biofilm infections.

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