Molecular Organization of Small Plasmids Bearing bla TEM-1 and Conferring Resistance to β-Lactams in Haemophilus influenzae | Zendy

Annette Søndergaard | Zendy; Álvaro San Millán | Zendy; Alfonso Santos-López | Zendy; Signe Maria Nielsen | Zendy; Bruno GonzálezZorn | Zendy; Niels NørskovLauritsen | Zendy

Open Access

Molecular Organization of Small Plasmids Bearing bla _TEM-1 and Conferring Resistance to β-Lactams in Haemophilus influenzae

Author(s) -

Annette Søndergaard,

Álvaro San Millán,

Alfonso Santos-López,

Signe Maria Nielsen,

Bruno GonzálezZorn,

Niels NørskovLauritsen

Publication year - 2012

Publication title -

antimicrobial agents and chemotherapy

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.07

H-Index - 259

eISSN - 1070-6283

pISSN - 0066-4804

DOI - 10.1128/aac.00408-12

Subject(s) - plasmid , haemophilus influenzae , biology , amp resistance , genetics , microbiology and biotechnology , gene , bacteria

TEM-1 is the dominant β-lactamase of Haemophilus influenzae and can be located on small plasmids. Three distinct plasmids with sizes from 4,304 to 5,646 nucleotides (nt) were characterized: pA1606, pA1209, and pPN223. In addition to TEM-1 and a replication enzyme of the Rep 3 superfamily, pA1606 carries a Tn3 resolvase gene and pA1606 and pA1209 carry an open reading frame (ORF) similar to a plasmid recombination enzyme gene described in Gram-positive bacteria. The plasmids transformed strain Rd to the ampicillin-resistant phenotype.

The content you want is available to Zendy users.

Already have an account? Click here to sign in.

Having issues? You can contact us here

Accelerating Research