Novel Small-Molecule Inhibitors of Transmissible Gastroenteritis Virus | Zendy

ChengWei Yang | Zendy; YungNing Yang | Zendy; PoHuang Liang | Zendy; ChiMin Chen | Zendy; WeiLiang Chen | Zendy; HwanYou Chang | Zendy; YuSheng Chao | Zendy; ShiowJu Lee | Zendy

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Novel Small-Molecule Inhibitors of Transmissible Gastroenteritis Virus

Author(s) -

ChengWei Yang,

YungNing Yang,

PoHuang Liang,

ChiMin Chen,

WeiLiang Chen,

HwanYou Chang,

YuSheng Chao,

ShiowJu Lee

Publication year - 2007

Publication title -

antimicrobial agents and chemotherapy

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.07

H-Index - 259

eISSN - 1070-6283

pISSN - 0066-4804

DOI - 10.1128/aac.00408-07

Subject(s) - virology , small molecule , virus , biology , microbiology and biotechnology , medicine , genetics

We used swine testicle (ST) cells infected with transmissible gastroenteritis virus (TGEV) and an indirect immunofluorescent assay with antibodies against TGEV spike and nucleocapsid proteins to screen small-molecule compounds that inhibit TGEV replication. Analogues of initial hits were collected and subjected to a 3CL protease (3CL(pro)) inhibition assay with recombinant 3CL(pro) and a fluorogenic peptide substrate. A series of benzothiazolium compounds were found to have inhibitory activity against TGEV 3CL(pro) and to exert anti-TGEV activities in terms of viral protein and RNA replication in TGEV-infected ST cells, with consequent protection of TGEV-infected ST cells from cytopathic effect by blocking the activation of caspase-3.

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