Open AccessAmodiaquine Resistance in Plasmodium falciparum Malaria in Afghanistan Is Associated with the pfcrt SVMNT Allele at Codons 72 to 76
Author(s) -
Khalid B. Beshir,
Colin J. Sutherland,
Ioannis Merinopoulos,
Naeem Durrani,
Toby Leslie,
Mark Rowland,
Rachel Hallett
Publication year - 2010
Publication title -
antimicrobial agents and chemotherapy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.07
H-Index - 259
eISSN - 1070-6283
pISSN - 0066-4804
DOI - 10.1128/aac.00358-10
Subject(s) - amodiaquine , plasmodium falciparum , malaria , virology , allele , biology , drug resistance , genetics , gene , immunology
Mutations in thePlasmodium falciparum genespfcrt andpfmdr1 are selected by amodiaquine treatment in Africa. To examine the importance of these mutations in amodiaquine-treated Asian parasites, we determined pre- and posttreatment genotypes for amodiaquine treatment failures from a clinical trial in Afghanistan. Thepfcrt codon 72 to 76 haplotype SVMNT was present in all samples tested, both before and after treatment. Amodiaquine did not clearly select for anypfmdr1 genotype, but a novel mutation,pfmdr1 N86F, was detected in four samples. We providein vivo data to support thein vitro correlation betweenpfcrt SVMNT and increased resistance to the metabolite of amodiaquine.