Multiplex Real-Time PCR for Detection of an Epidemic KPC-Producing Klebsiella pneumoniae ST258 Clone | Zendy

Liang Chen | Zendy; Kalyan D. Chavda | Zendy; José R. Mediavilla | Zendy; Yanan Zhao | Zendy; Henry Fraimow | Zendy; Stephen G. Jenkins | Zendy; Michael H. Levi | Zendy; Tao Hong | Zendy; Albert Rojtman | Zendy; Christine C. Ginocchio | Zendy; Robert A. Bonomo | Zendy; Barry N. Kreiswirth | Zendy

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Multiplex Real-Time PCR for Detection of an Epidemic KPC-Producing Klebsiella pneumoniae ST258 Clone

Author(s) -

Liang Chen,

Kalyan D. Chavda,

José R. Mediavilla,

Yanan Zhao,

Henry Fraimow,

Stephen G. Jenkins,

Michael H. Levi,

Tao Hong,

Albert Rojtman,

Christine C. Ginocchio,

Robert A. Bonomo,

Barry N. Kreiswirth

Publication year - 2012

Publication title -

antimicrobial agents and chemotherapy

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.07

H-Index - 259

eISSN - 1070-6283

pISSN - 0066-4804

DOI - 10.1128/aac.00316-12

Subject(s) - klebsiella pneumoniae , clone (java method) , multiplex , multiplex polymerase chain reaction , microbiology and biotechnology , virology , biology , polymerase chain reaction , gene , escherichia coli , bioinformatics , genetics

We describe a multiplex real-time PCR assay capable of identifying both the epidemic Klebsiella pneumoniae ST258 clone and bla(KPC) carbapenemase genes in a single reaction. The assay displayed excellent sensitivity (100%) and specificity (100%) for identification of ST258 clone and bla(KPC) in a collection of 75 K. pneumoniae isolates comprising 41 sequence types. Our results suggest that this assay is an effective tool for surveillance of this clone among carbapenem-resistant K. pneumoniae clinical isolates.

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