Open AccessMeasurement of Voriconazole Activity against Candida albicans , C. glabrata , and C. parapsilosis Isolates Using Time-Kill Methods Validated by High-Performance Liquid Chromatography
Author(s) -
Yanjun Li,
M. Hong Nguyen,
Hartmut Derendorf,
Shaoji Cheng,
Cornelius J. Clancy
Publication year - 2007
Publication title -
antimicrobial agents and chemotherapy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.07
H-Index - 259
eISSN - 1070-6283
pISSN - 0066-4804
DOI - 10.1128/aac.00308-07
Subject(s) - voriconazole , candida albicans , high performance liquid chromatography , candida parapsilosis , candida glabrata , microbiology and biotechnology , corpus albicans , biology , chromatography , chemistry , antifungal
We developed a high-performance liquid chromatography (HPLC) assay to validate time-kill and postantifungal-effect (PAFE) experiments for voriconazole againstCandida albicans ,Candida glabrata , andCandida parapsilosis isolates. Voriconazole exerted prolonged fungistatic activity but no PAFE at concentrations achievable in human sera. HPLC confirmed that experiments were conducted at the desired steady-state voriconazole concentrations.