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ACH-702 is a new isothiazoloquinolone with potentin vitro andin vivo activities against important bacterial pathogens, includingStaphylococcus aureus. In this study, ACH-702 was found to have promisingin vitro antibacterial activity againstMycobacterium tuberculosis , with MICs of ≤1 μg/ml, comparable to that of the fluoroquinolone moxifloxacin for quinolone-susceptible isolates but superior to that for quinolone-resistant isolates. Biochemical assays involvingM. tuberculosis gyrase enzymes indicated that ACH-702 had significantly improved inhibitory activity compared with fluoroquinolones.