Update to the Multiplex PCR Strategy for Assignment of mec Element Types in Staphylococcus aureus | Zendy

Catarina Milheiriço | Zendy; Duarte C. Oliveira | Zendy; Hermı́nia de Lencastre | Zendy

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Update to the Multiplex PCR Strategy for Assignment of mec Element Types in Staphylococcus aureus

Author(s) -

Catarina Milheiriço,

Duarte C. Oliveira,

Hermı́nia de Lencastre

Publication year - 2007

Publication title -

antimicrobial agents and chemotherapy

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.07

H-Index - 259

eISSN - 1070-6283

pISSN - 0066-4804

DOI - 10.1128/aac.00275-07

Subject(s) - sccmec , typing , multiplex polymerase chain reaction , staphylococcus aureus , multiplex , biology , microbiology and biotechnology , genetics , computational biology , polymerase chain reaction , methicillin resistant staphylococcus aureus , gene , bacteria

Staphylococcal cassette chromosome mec (SCCmec) typing is important for the identification and definition of methicillin-resistant Staphylococcus aureus clones, and for routine purposes, multiplex PCR assays are the most adequate for SCCmec typing. Here, we describe an update to the multiplex PCR strategy for SCCmec typing that we described in 2002 so that SCCmec types IV and V may be properly identified.

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