Staphylococcus aureus Cell Wall Stress Stimulon Gene -lacZ Fusion Strains: Potential for Use in Screening for Cell Wall-Active Antimicrobials | Zendy

Rebecca J. Steidl | Zendy; Stacy L. Pearson | Zendy; Robert E. Stephenson | Zendy; Nagender Ledala | Zendy; Sutthirat Sitthisak | Zendy; Brian J. Wilkinson | Zendy; Radheshyam K. Jayaswal | Zendy

Open Access

Staphylococcus aureus Cell Wall Stress Stimulon Gene -lacZ Fusion Strains: Potential for Use in Screening for Cell Wall-Active Antimicrobials

Author(s) -

Rebecca J. Steidl,

Stacy L. Pearson,

Robert E. Stephenson,

Nagender Ledala,

Sutthirat Sitthisak,

Brian J. Wilkinson,

Radheshyam K. Jayaswal

Publication year - 2008

Publication title -

antimicrobial agents and chemotherapy

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.07

H-Index - 259

eISSN - 1070-6283

pISSN - 0066-4804

DOI - 10.1128/aac.00273-08

Subject(s) - cell wall , staphylococcus aureus , microbiology and biotechnology , biology , fusion gene , cell , gene , antimicrobial , lac operon , antibiotics , cell fusion , escherichia coli , bacteria , genetics

lacZ fusion strains were constructed using the promoters of five cell wall stress stimulon genes: pbp2, tcaA, vraSR, sgtB, and lytR. All fusion strains were induced only in the presence of cell wall-active antibiotics, suggesting the potential of these strains for use in high-throughput screening for new cell wall-active agents.

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