Inducible and Constitutive Activation of Two Polymorphic Promoter Alleles of the Candida albicans Multidrug Efflux Pump MDR1 | Zendy

Christoph Sasse | Zendy; Rebecca Schillig | Zendy; Alexandra Reimund | Zendy; Julia Merk | Zendy; Joachim Morschhäuser | Zendy

Open Access

Inducible and Constitutive Activation of Two Polymorphic Promoter Alleles of the Candida albicans Multidrug Efflux Pump MDR1

Author(s) -

Christoph Sasse,

Rebecca Schillig,

Alexandra Reimund,

Julia Merk,

Joachim Morschhäuser

Publication year - 2012

Publication title -

antimicrobial agents and chemotherapy

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.07

H-Index - 259

eISSN - 1070-6283

pISSN - 0066-4804

DOI - 10.1128/aac.00264-12

Subject(s) - efflux , candida albicans , fluconazole , biology , allele , multiple drug resistance , microbiology and biotechnology , corpus albicans , locus (genetics) , drug resistance , genetics , loss of heterozygosity , gene , antifungal

Overexpression of the multidrug efflux pump MDR1 confers resistance to the antifungal drug fluconazole on Candida albicans. It has been reported that two types of MDR1 promoters exist in C. albicans and that homozygosity for the allele with higher activity may promote fluconazole resistance. We found that the two MDR1 promoter alleles in strain SC5314 were equally well activated by inducing chemicals or hyperactive forms of the transcription factors Mrr1 and Cap1, which control MDR1 expression. In addition, no loss of heterozygosity at the MDR1 locus was observed in MDR1-overexpressing clinical C. albicans strains that developed fluconazole resistance during therapy.

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