New Small Plasmid Harboring bla KPC-2 in Pseudomonas aeruginosa | Zendy

Renata Galetti | Zendy; Leonardo Neves Andrade | Zendy; Michaël Chandler | Zendy; Alessandro M. Varani | Zendy; Ana Lúcia da Costa Darini | Zendy

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New Small Plasmid Harboring bla _KPC-2 in Pseudomonas aeruginosa

Author(s) -

Renata Galetti,

Leonardo Neves Andrade,

Michaël Chandler,

Alessandro M. Varani,

Ana Lúcia da Costa Darini

Publication year - 2016

Publication title -

antimicrobial agents and chemotherapy

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.07

H-Index - 259

eISSN - 1070-6283

pISSN - 0066-4804

DOI - 10.1128/aac.00247-16

Subject(s) - pseudomonas aeruginosa , plasmid , biology , tn3 transposon , escherichia coli , genetics , context (archaeology) , strain (injury) , gene , microbiology and biotechnology , transposable element , inverted repeat , genome , bacteria , paleontology , anatomy

The aim of this study was to characterize the genetic context ofbla KPC-2 inPseudomonas aeruginosa sequence type 244 from Brazil. Thebla KPC-2 gene was detected in a new small plasmid, pBH6. Complete sequencing revealed that pBH6 was 3,652 bp long and included the Tn3 resolvase and Tn3 inverted repeat (IR), a partial copy of ISKpn6 , and a putativeori region but norep genes. pBH6 replicated stably intoEscherichia coli strain DH10B andP. aeruginosa strain PAO.

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