Open AccessMolecular Drug Susceptibility Testing and Genotyping of Mycobacterium leprae Strains from South America
Author(s) -
Pushpendra Singh,
Philippe Busso,
Alberto PanizMondolfi,
Nacarid Aránzazu,
Marc Monot,
Nadine Honoré,
Andréa de Faria Fernandes Belone,
Marcos Virmond,
Maria Esther Villarreal-Olaya,
Carlos Rivas,
Stewart T. Cole
Publication year - 2011
Publication title -
antimicrobial agents and chemotherapy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.07
H-Index - 259
eISSN - 1070-6283
pISSN - 0066-4804
DOI - 10.1128/aac.00201-11
Subject(s) - genotyping , mycobacterium leprae , dapsone , leprosy , drug resistance , biology , genotype , microbiology and biotechnology , virology , genetics , gene , immunology
Possible drug resistance in Mycobacterium leprae strains from Venezuela and three other South American countries was surveyed by molecular methods. None of the 230 strains from new leprosy cases exhibited drug resistance-associated mutations. However, two of the three strains from relapsed cases contained dapsone resistance mutations, and one strain also harbored a rifampin resistance mutation. Single nucleotide polymorphism analysis of these strains revealed five subtypes: 3I (73.8%), 4P (11.6%), 1D (6.9%), 4N (6%), and 4O (1.7%).