Evaluation of Target Specificity of Antibacterial Agents Using Staphylococcus aureus ddlA Mutants and d -Cycloserine in a Silkworm Infection Model | Zendy

Kenji Kurokawa | Zendy; Hiroshi Hamamoto | Zendy; Miki Matsuo | Zendy; Satoshi Nishida | Zendy; Noriko Yamane | Zendy; Yong Seok Lee | Zendy; Kazuhisa Murakami | Zendy; Hideki Maki | Zendy; Kazuhisa Sekimizu | Zendy

Open Access

Evaluation of Target Specificity of Antibacterial Agents Using Staphylococcus aureus ddlA Mutants and d -Cycloserine in a Silkworm Infection Model

Author(s) -

Kenji Kurokawa,

Hiroshi Hamamoto,

Miki Matsuo,

Satoshi Nishida,

Noriko Yamane,

Yong Seok Lee,

Kazuhisa Murakami,

Hideki Maki,

Kazuhisa Sekimizu

Publication year - 2009

Publication title -

antimicrobial agents and chemotherapy

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.07

H-Index - 259

eISSN - 1070-6283

pISSN - 0066-4804

DOI - 10.1128/aac.00200-09

Subject(s) - cycloserine , staphylococcus aureus , antibiotics , microbiology and biotechnology , ed50 , biology , mutant , antibacterial agent , micrococcaceae , antibacterial activity , bacteria , in vitro , biochemistry , genetics , gene

The availability of a silkworm larva infection model to evaluate the therapeutic effectiveness of antibiotics was examined. The 50% effective doses (ED50) of D-cycloserine against the Staphylococcus aureus ddlA mutant-mediated killing of larvae were remarkably lower than those against the parental strain-mediated killing of larvae. Changes in MICs and ED50 of other antibiotics were negligible, suggesting that these alterations are d-cycloserine selective. Therefore, this model is useful for selecting desired compounds based on their therapeutic effectiveness during antibiotic development.

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