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We expressed the wild-typerplC and mutatedrplC (Cys154Arg) genes, respectively, inMycobacterium tuberculosis H37Ra and H37Rv in an attempt to delineate the role ofrplC (Cys154Arg) regarding oxazolidinone resistance. An increase of the MICs of linezolid (LZD) and sutezolid (PNU-100480, PNU) against the recombinant mycobacteria with overexpressedrplC mutation (Cys154Arg) was found, suggesting therplC gene is a determinant of bacillary susceptibilities to LZD and PNU.

Role of the Cys154Arg Substitution in Ribosomal Protein L3 in Oxazolidinone Resistance in Mycobacterium tuberculosis