Mutational Effects on Carbapenem Hydrolysis of YEM-1, a New Subclass B2 Metallo-β-Lactamase from Yersinia mollaretii | Zendy

Paola Sandra Mercuri | Zendy; Roberto Esposito | Zendy; Sylvie Blétard | Zendy; Stefano Costanzo | Zendy; Mariagrazia Perilli | Zendy; Frédéric Kerff | Zendy; Moreno Galleni | Zendy

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Mutational Effects on Carbapenem Hydrolysis of YEM-1, a New Subclass B2 Metallo-β-Lactamase from Yersinia mollaretii

Author(s) -

Paola Sandra Mercuri,

Roberto Esposito,

Sylvie Blétard,

Stefano Costanzo,

Mariagrazia Perilli,

Frédéric Kerff,

Moreno Galleni

Publication year - 2020

Publication title -

antimicrobial agents and chemotherapy

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.07

H-Index - 259

eISSN - 1070-6283

pISSN - 0066-4804

DOI - 10.1128/aac.00105-20

Subject(s) - subclass , microbiology and biotechnology , carbapenem , enterobacteriaceae , biology , chemistry , escherichia coli , antibiotics , biochemistry , genetics , antibody , gene

Analysis of the genome sequence ofYersinia mollaretii ATCC 43969 identified thebla YEM gene, encoding YEM-1, a putative subclass B2 metallo-β-lactamase. The objectives of our work were to produce and purify YEM-1 and to complete its kinetic characterization. YEM-1 displayed the narrowest substrate range among known subclass B2 metallo-β-lactamases, since it can hydrolyze imipenem, but not other carbapenems, such as biapenem, meropenem, doripenem, and ertapenem, with high catalytic efficiency.

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