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A novel transposon belonging to the Tn 3 -like family was identified on the chromosome of a commensal strain of Pseudomonas aeruginosa sequence type 2343 (ET02). Tn 6350 is 7,367 bp long and harbors eight open reading frames (ORFs), an ATPase (IS 481 family), a transposase (DDE catalytic type), a Tn 3 resolvase, three hypothetical proteins, and genes encoding the new pyocin S8 with its immunity protein. We show that pyocin S8 displays activity against carbapenemase-producing P. aeruginosa , including IMP-1, SPM-1, VIM-1, GES-5, and KPC-2 producers.

antimicrobial agents and chemotherapy

Tn <i>6350</i> , a Novel Transposon Carrying Pyocin S8 Genes Encoding a Bacteriocin with Activity against Carbapenemase-Producing Pseudomonas aeruginosa

Tn 6350 , a Novel Transposon Carrying Pyocin S8 Genes Encoding a Bacteriocin with Activity against Carbapenemase-Producing Pseudomonas aeruginosa