Open AccessSugE, a New Member of the SMR Family of Transporters, Contributes to Antimicrobial Resistance in Enterobacter cloacae
Author(s) -
Gui-Xin He,
Chu Zhang,
Robert R. Crow,
Conner Thorpe,
Huizhong Chen,
Sanath Kumar,
Tomofusa Tsuchiya,
Manuel F. Varela
Publication year - 2011
Publication title -
antimicrobial agents and chemotherapy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.07
H-Index - 259
eISSN - 1070-6283
pISSN - 0066-4804
DOI - 10.1128/aac.00094-11
Subject(s) - enterobacter cloacae , microbiology and biotechnology , antimicrobial , enterobacter , antibiotic resistance , enterobacteriaceae infections , biology , enterobacteriaceae , genetics , antibiotics , gene , escherichia coli
We cloned a gene,sugE , from the chromosome ofEnterobacter cloacae ATCC 13047. Analysis of the susceptibilities of thesugE -containing strain (Escherichia coli KAM32/pSUGE28) andsugE -deficientE. cloacae (Ec ΔsugE ) showed that SugE confers resistance to cetyltrimethylammonium bromide, cetylpyridinium chloride, tetraphenylphosphonium, benzalkonium chloride, ethidium bromide, and sodium dodecyl sulfate. We also investigated expression ofsugE . We confirm here that SugE fromE. cloacae is an SMR family transporter as determined by observing its energy-dependent drug efflux activity.