Open AccessMolecular Basis of Macrolide, Triamilide, and Lincosamide Resistance in Pasteurella multocida from Bovine Respiratory Disease
Author(s) -
Kristina Kadlec,
Geovana Brenner Michael,
Michael T. Sweeney,
Elżbieta Brzuszkiewicz,
Heiko Liesegang,
Rolf Daniel,
Jeffrey L. Watts,
Štefan Schwarz
Publication year - 2011
Publication title -
antimicrobial agents and chemotherapy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.07
H-Index - 259
eISSN - 1070-6283
pISSN - 0066-4804
DOI - 10.1128/aac.00092-11
Subject(s) - pasteurella multocida , biology , microbiology and biotechnology , gene , plasmid , escherichia coli , genetics , bacteria
The mechanism of macrolide-triamilide resistance in Pasteurella multocida has been unknown. During whole-genome sequencing of a multiresistant bovine P. multocida isolate, three new resistance genes, the rRNA methylase gene erm(42), the macrolide transporter gene msr(E), and the macrolide phosphotransferase gene mph(E), were detected. The three genes were PCR amplified, cloned into suitable plasmid vectors, and shown to confer either macrolide-lincosamide resistance [erm(42)] or macrolide-triamilide resistance [msr(E)-mph(E)] in macrolide-susceptible Escherichia coli and P. multocida hosts.