Open AccessReal-Time PCR Detection of gyrA and parC Mutations in Streptococcus pneumoniae
Author(s) -
Stéphanie Le Page,
Frédérique Vernel-Pauillac,
Olivia O’Connor,
Sylvie Brémont,
Françoise Charavay,
Patrice Courvalin,
Cyrille Goarant,
Simon Le Hello
Publication year - 2008
Publication title -
antimicrobial agents and chemotherapy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.07
H-Index - 259
eISSN - 1070-6283
pISSN - 0066-4804
DOI - 10.1128/aac.00082-08
Subject(s) - streptococcus pneumoniae , biology , microbiology and biotechnology , quinolone , mutation , streptococcaceae , gene , virology , genetics , antibiotics
Fluoroquinolone resistance in Streptococcus pneumoniae mainly involves stepwise mutations predominantly in the parC and gyrA genes. We have developed a single-run real-time PCR assay for detection of the four most common mutations in the quinolone resistance-determining regions of these genes. This assay provides a useful tool for both clinical and epidemiological use.