Open AccessThe widespread IS200/IS605 transposon family encodes diverse programmable RNA-guided endonucleases
Author(s) -
Han Altae-Tran,
Soumya Kannan,
F. Esra Demircioglu,
Rachel Oshiro,
Suchita P. Nety,
Luke J. McKay,
Mensur Dlakić,
William P. Inskeep,
Kira S. Makarova,
Rhian K. Macrae,
Eugene V. Koonin,
Feng Zhang
Publication year - 2021
Publication title -
science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 12.556
H-Index - 1186
eISSN - 1095-9203
pISSN - 0036-8075
DOI - 10.1126/science.abj6856
Subject(s) - transposable element , genetics , rna , biology , crispr , endonuclease , cas9 , genome , computational biology , nuclease , rna editing , dna , gene
Tracing the origin of CRISPR-Cas CRISPR-Cas systems have transformed genome editing and other biotechnologies; however, the broader origins and diversity of RNA-guided nucleases have largely remained unexplored. Altae-Tranet al . show that three distinct transposon-encoded proteins, IscB, IsrB, and TnpB, are naturally occurring, reprogrammable RNA-guided DNA nucleases (see the Perspective by Rousset and Sorek). In addition to identifying diverse guide-encoding mechanisms, the authors elucidate the evolutionary relationship between IsrB, IscB, and CRISPR-Cas9. Overall, these newly characterized systems, called OMEGA (for obligate mobile element–guided activity) systems, are found in all domains of life and may be harnessed for biotechnology development. —DJ
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