Open AccessTime-resolved structured illumination microscopy reveals key principles of Xist RNA spreading
Author(s) -
Lisa Rodermund,
Heather Coker,
Roel Oldenkamp,
Guifeng Wei,
Joseph S. Bowness,
Bramman Rajkumar,
Tatyana B. Nesterova,
David Miguel Susano Pinto,
Lothar Schermelleh,
Neil Brockdorff
Publication year - 2021
Publication title -
science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 12.556
H-Index - 1186
eISSN - 1095-9203
pISSN - 0036-8075
DOI - 10.1126/science.abe7500
Subject(s) - xist , key (lock) , microscopy , rna , computational biology , biology , microbiology and biotechnology , computer science , chemistry , optics , biophysics , x inactivation , physics , genetics , x chromosome , ecology , gene
Visualizing Xist RNA dynamics The noncoding RNA Xist, which controls the process of X chromosome inactivation in mammals, accumulates and spreads over the chromosome from which it is transcribed. The underlying basis for this unusual behavior is poorly understood. Using a new imaging approach called RNA-SPLIT for time-resolved analysis of Xist RNA molecules at super-resolution, Rodermundet al. analyzed fundamental parameters of Xist RNA behavior in normal cells and after the perturbation of factors implicated in Xist RNA function. The authors provide new insights into the basis of Xist RNA localization and confinement within the territory of a single X chromosome.Science , abe7500, this issue p.eabe7500
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