Antibody cocktail to SARS-CoV-2 spike protein prevents rapid mutational escape seen with individual antibodies | Zendy

Alina Baum | Zendy; Benjamin O. Fulton | Zendy; Elzbieta Wloga | Zendy; Richard Copin | Zendy; Kristen E. Pascal | Zendy; Vincenzo Russo | Zendy; Stephanie Giordano | Zendy; Kathryn Lanza | Zendy; Nicole Negron | Zendy; Min Ni | Zendy; Yi Wei | Zendy; Gurinder S. Atwal | Zendy; Andrew Murphy | Zendy; Neil Stahl | Zendy; George D. Yancopoulos | Zendy; Christos A. Kyratsous | Zendy

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Antibody cocktail to SARS-CoV-2 spike protein prevents rapid mutational escape seen with individual antibodies

Author(s) -

Alina Baum,

Benjamin O. Fulton,

Elzbieta Wloga,

Richard Copin,

Kristen E. Pascal,

Vincenzo Russo,

Stephanie Giordano,

Kathryn Lanza,

Nicole Negron,

Min Ni,

Yi Wei,

Gurinder S. Atwal,

Andrew Murphy,

Neil Stahl,

George D. Yancopoulos,

Christos A. Kyratsous

Publication year - 2020

Publication title -

science

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 12.556

H-Index - 1186

eISSN - 1095-9203

pISSN - 0036-8075

DOI - 10.1126/science.abd0831

Subject(s) - antibody , spike protein , virology , spike (software development) , biology , covid-19 , neutralization , mutant , coronavirus , population , immunology , gene , medicine , genetics , disease , infectious disease (medical specialty) , management , pathology , economics , environmental health

Antibodies targeting the spike protein of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) present a promising approach to combat the coronavirus disease 2019 (COVID-19) pandemic; however, concerns remain that mutations can yield antibody resistance. We investigated the development of resistance against four antibodies to the spike protein that potently neutralize SARS-CoV-2, individually as well as when combined into cocktails. These antibodies remain effective against spike variants that have arisen in the human population. However, novel spike mutants rapidly appeared after in vitro passaging in the presence of individual antibodies, resulting in loss of neutralization; such escape also occurred with combinations of antibodies binding diverse but overlapping regions of the spike protein. Escape mutants were not generated after treatment with a noncompeting antibody cocktail.

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