In situ genome sequencing resolves DNA sequence and structure in intact biological samples | Zendy

Andrew C. Payne | Zendy; Zachary Chiang | Zendy; Paul Reginato | Zendy; Sarah Mangiameli | Zendy; Evan Murray | Zendy; ChunChen Yao | Zendy; Styliani Markoulaki | Zendy; Andrew Earl | Zendy; Ajay Labade | Zendy; Rudolf Jaenisch | Zendy; George M. Church | Zendy; Edward S. Boyden | Zendy; Jason D. Buenrostro | Zendy; Fei Chen | Zendy

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In situ genome sequencing resolves DNA sequence and structure in intact biological samples

Author(s) -

Andrew C. Payne,

Zachary Chiang,

Paul Reginato,

Sarah Mangiameli,

Evan Murray,

ChunChen Yao,

Styliani Markoulaki,

Andrew Earl,

Ajay Labade,

Rudolf Jaenisch,

George M. Church,

Edward S. Boyden,

Jason D. Buenrostro,

Fei Chen

Publication year - 2021

Publication title -

science

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 12.556

H-Index - 1186

eISSN - 1095-9203

pISSN - 0036-8075

DOI - 10.1126/science.aay3446

Subject(s) - genome , chromatin , biology , computational biology , chromosome conformation capture , in situ , genetics , human genome , chromosome , dna sequencing , sequence (biology) , dna , gene , gene expression , physics , enhancer , meteorology

Visualizing the 3D genome in situ The conformation of the genome within the cell changes depending on cell state, such that being able to visualize genome structure can identify cis and trans interactions among regulatory genetic elements. Payneet al. have developed an unbiased genome-sequencing technique in single cells in situ that can infer the chromatin structure by imaging. They were able to identify sequences at subnuclei locations to analyze the proximity relationships among genetic elements within and across chromosomes in single cells. Using this technique, they could detect chromosome territories and distinctions between different types of repetitive sequences and chromosomal features. This method can map and image genomic coordinates with submicrometer resolution in intact single cells.Science , this issue p.eaay3446

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