How a circularized tmRNA moves through the ribosome | Zendy

Christopher D. Rae | Zendy; Yuliya Gordiyenko | Zendy; V. Ramakrishnan | Zendy

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How a circularized tmRNA moves through the ribosome

Author(s) -

Christopher D. Rae,

Yuliya Gordiyenko,

V. Ramakrishnan

Publication year - 2019

Publication title -

science

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 12.556

H-Index - 1186

eISSN - 1095-9203

pISSN - 0036-8075

DOI - 10.1126/science.aav9370

Subject(s) - ribosome , messenger rna , translation (biology) , microbiology and biotechnology , rna , chemistry , p site , transfer rna , ef tu , protein biosynthesis , biology , biochemistry , gene

During trans-translation, transfer-messenger RNA (tmRNA) and small protein B (SmpB) together rescue ribosomes stalled on a truncated mRNA and tag the nascent polypeptide for degradation. We used cryo-electron microscopy to determine the structures of three key states of the tmRNA-SmpB-ribosome complex during trans translation at resolutions of 3.7 to 4.4 angstroms. The results show how tmRNA and SmpB act specifically on stalled ribosomes and how the circularized complex moves through the ribosome, enabling translation to switch from the old defective message to the reading frame on tmRNA.

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