Programmed DNA destruction by miniature CRISPR-Cas14 enzymes | Zendy

Lucas B. Harrington | Zendy; David Burstein | Zendy; Janice S. Chen | Zendy; David Páez-Espino | Zendy; Enbo Ma | Zendy; Isaac P. Witte | Zendy; Joshua C. Cofsky | Zendy; Nikos C. Kyrpides | Zendy; Jillian F. Banfield | Zendy; Jennifer A. Doudna | Zendy

AI Assistant Blog Pricing

Home ZAIA Blog

Open Access

Programmed DNA destruction by miniature CRISPR-Cas14 enzymes

Author(s) -

Lucas B. Harrington,

David Burstein,

Janice S. Chen,

David Páez-Espino,

Enbo Ma,

Isaac P. Witte,

Joshua C. Cofsky,

Nikos C. Kyrpides,

Jillian F. Banfield,

Jennifer A. Doudna

Publication year - 2018

Publication title -

science

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 12.556

H-Index - 1186

eISSN - 1095-9203

pISSN - 0036-8075

DOI - 10.1126/science.aav4294

Subject(s) - crispr , biology , rna , nucleic acid , dna , computational biology , effector , metagenomics , genetics , gene , biochemistry

A programmable type of CRISPR system CRISPR-Cas9 systems have been causing a revolution in biology. Harringtonet al. describe the discovery and technological implementation of an additional type of CRISPR system based on an extracompact effector protein, Cas14. Metagenomics data, particularly from uncultivated samples, uncovered the CRISPR-Cas14 systems containing all the components necessary for adaptive immunity in prokaryotes. At half the size of class 2 CRISPR effectors, Cas14 appears to target single-stranded DNA without class 2 sequence restrictions. By leveraging this activity, a fast and high-fidelity nucleic acid detection system enabled detection of single-nucleotide polymorphisms.Science , this issue p.839

The content you want is available to Zendy users.

Already have an account? Click here to sign in.

Having issues? You can contact us here

Accelerating Research