Discovery of widespread type I and type V CRISPR-Cas inhibitors | Zendy

Nicole D. Marino | Zendy; Jenny Y. Zhang | Zendy; Adair L. Borges | Zendy; Alexander A. Sousa | Zendy; Lina M. León | Zendy; Benjamin J. Rauch | Zendy; Russell T. Walton | Zendy; Joel Berry | Zendy; J. Keith Joung | Zendy; Benjamin P. Kleinstiver | Zendy; Joseph BondyDenomy | Zendy

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Discovery of widespread type I and type V CRISPR-Cas inhibitors

Author(s) -

Nicole D. Marino,

Jenny Y. Zhang,

Adair L. Borges,

Alexander A. Sousa,

Lina M. León,

Benjamin J. Rauch,

Russell T. Walton,

Joel Berry,

J. Keith Joung,

Benjamin P. Kleinstiver,

Joseph BondyDenomy

Publication year - 2018

Publication title -

science

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 12.556

H-Index - 1186

eISSN - 1095-9203

pISSN - 0036-8075

DOI - 10.1126/science.aau5174

Subject(s) - crispr , type (biology) , computational biology , biology , genetics , gene , ecology

Cas12 inhibitors join the anti-CRISPR family Bacteria and their phages continually coevolve in a molecular arms race. For example, phages use anti-CRISPR proteins to inhibit the bacterial type I and II CRISPR systems (see the Perspective by Koonin and Makarova). Watterset al. and Marinoet al. used bioinformatic and experimental approaches to identify inhibitors of type V CRISPR-Cas12a. Cas12a has been successfully engineered for gene editing and nucleic acid detection. Some of the anti-Cas12a proteins identified in these studies had broad-spectrum inhibitory effects on Cas12a orthologs and could block Cas12a-mediated genome editing in human cells.Science , this issue p.236 , p.240 ; see also p.156

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