Open AccessSubcellular antibiotic visualization reveals a dynamic drug reservoir in infected macrophages
Author(s) -
Daniel J. Greenwood,
Mariana Silva dos Santos,
Song Huang,
Matthew R. G. Russell,
Lucy Collinson,
James I. MacRae,
Andy West,
Haibo Jiang,
Maximiliano G. Gutiérrez
Publication year - 2019
Publication title -
science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 12.556
H-Index - 1186
eISSN - 1095-9203
pISSN - 0036-8075
DOI - 10.1126/science.aat9689
Subject(s) - bedaquiline , antibiotics , intracellular , bacilli , microbiology and biotechnology , lipid droplet , intracellular parasite , bacteria , drug , biology , host (biology) , chemistry , tuberculosis , pharmacology , medicine , mycobacterium tuberculosis , pathology , ecology , genetics
Lipid droplets help anti-TB drug efficacy Improving chemotherapies against intracellular pathogens requires understanding how antibiotic distribution within infected cells affects efficacy. Greenwoodet al. developed an approach to visualize antibiotics in human macrophages infected with the tubercle bacillus (see the Perspective by Smith and Aldridge). They showed that the antitubercular (anti-TB) drug bedaquiline accumulated in host lipid droplets. Lipid droplets seemed to act as an antibiotic reservoir that could be transferred to bacteria during host lipid consumption. Indeed, alterations in host lipid droplet content affected the anti-TB activity of bedaquiline against intracellular bacilli.Science , this issue p.1279 ; see also p.1234
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