A method for single-neuron chronic recording from the retina in awake mice | Zendy

Guosong Hong | Zendy; Tian-Ming Fu | Zendy; Mu Qiao | Zendy; Robert D. Viveros | Zendy; Xiao Yang | Zendy; Tao Zhou | Zendy; Jung Min Lee | Zendy; HongGyu Park | Zendy; Joshua R. Sanes | Zendy; Charles M. Lieber | Zendy

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A method for single-neuron chronic recording from the retina in awake mice

Author(s) -

Guosong Hong,

Tian-Ming Fu,

Mu Qiao,

Robert D. Viveros,

Xiao Yang,

Tao Zhou,

Jung Min Lee,

HongGyu Park,

Joshua R. Sanes,

Charles M. Lieber

Publication year - 2018

Publication title -

science

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 12.556

H-Index - 1186

eISSN - 1095-9203

pISSN - 0036-8075

DOI - 10.1126/science.aas9160

Subject(s) - retina , retinal implant , neuroscience , retinal , ganglion , intrinsically photosensitive retinal ganglion cells , circadian rhythm , giant retinal ganglion cells , neuron , retinal ganglion cell , retinal waves , biology , anatomy , medicine , ophthalmology

The retina, which processes visual information and sends it to the brain, is an excellent model for studying neural circuitry. It has been probed extensively ex vivo but has been refractory to chronic in vivo electrophysiology. We report a nonsurgical method to achieve chronically stable in vivo recordings from single retinal ganglion cells (RGCs) in awake mice. We developed a noncoaxial intravitreal injection scheme in which injected mesh electronics unrolls inside the eye and conformally coats the highly curved retina without compromising normal eye functions. The method allows 16-channel recordings from multiple types of RGCs with stable responses to visual stimuli for at least 2 weeks, and reveals circadian rhythms in RGC responses over multiple day/night cycles.

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