ppGpp couples transcription to DNA repair in E. coli | Zendy

Venu Kamarthapu | Zendy; Vitaly Epshtein | Zendy; Bradley Benjamin | Zendy; Sergey Proshkin | Zendy; А. С. Миронов | Zendy; Michael Cashel | Zendy; Evgeny Nudler | Zendy

AI Assistant Blog Pricing

Home ZAIA Blog

Open Access

ppGpp couples transcription to DNA repair in E. coli

Author(s) -

Venu Kamarthapu,

Vitaly Epshtein,

Bradley Benjamin,

Sergey Proshkin,

А. С. Миронов,

Michael Cashel,

Evgeny Nudler

Publication year - 2016

Publication title -

science

Language(s) - Uncategorized

Resource type - Journals

SCImago Journal Rank - 12.556

H-Index - 1186

eISSN - 1095-9203

pISSN - 0036-8075

DOI - 10.1126/science.aad6945

Subject(s) - stringent response , dna repair , dna , transcription (linguistics) , rna polymerase , biology , dna damage , nucleotide excision repair , microbiology and biotechnology , escherichia coli , genetics , gene , linguistics , philosophy

The small molecule alarmone (p)ppGpp mediates bacterial adaptation to nutrient deprivation by altering the initiation properties of RNA polymerase (RNAP). ppGpp is generated in Escherichia coli by two related enzymes, RelA and SpoT. We show that ppGpp is robustly, but transiently, induced in response to DNA damage and is required for efficient nucleotide excision DNA repair (NER). This explains why relA-spoT-deficient cells are sensitive to diverse genotoxic agents and ultraviolet radiation, whereas ppGpp induction renders them more resistant to such challenges. The mechanism of DNA protection by ppGpp involves promotion of UvrD-mediated RNAP backtracking. By rendering RNAP backtracking-prone, ppGpp couples transcription to DNA repair and prompts transitions between repair and recovery states.

The content you want is available to Zendy users.

Already have an account? Click here to sign in.

Having issues? You can contact us here

Accelerating Research