Ubiquitin facilitates a quality-control pathway that removes damaged chloroplasts | Zendy

Jesse D. Woodson | Zendy; Matthew S. Joens | Zendy; Andrew B. Sinson | Zendy; Jonathan Gilkerson | Zendy; Patrice A. Salomé | Zendy; Detlef Weigel | Zendy; James A. J. Fitzpatrick | Zendy; Joanne Chory | Zendy

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Ubiquitin facilitates a quality-control pathway that removes damaged chloroplasts

Author(s) -

Jesse D. Woodson,

Matthew S. Joens,

Andrew B. Sinson,

Jonathan Gilkerson,

Patrice A. Salomé,

Detlef Weigel,

James A. J. Fitzpatrick,

Joanne Chory

Publication year - 2015

Publication title -

science

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 12.556

H-Index - 1186

eISSN - 1095-9203

pISSN - 0036-8075

DOI - 10.1126/science.aac7444

Subject(s) - chloroplast , ubiquitin ligase , arabidopsis , reactive oxygen species , microbiology and biotechnology , mutant , ubiquitin , biology , photosynthesis , oxidative stress , biochemistry , chemistry , gene

Energy production by chloroplasts and mitochondria causes constant oxidative damage. A functioning photosynthetic cell requires quality-control mechanisms to turn over and degrade chloroplasts damaged by reactive oxygen species (ROS). Here, we generated a conditionally lethal Arabidopsis mutant that accumulated excess protoporphyrin IX in the chloroplast and produced singlet oxygen. Damaged chloroplasts were subsequently ubiquitinated and selectively degraded. A genetic screen identified the plant U-box 4 (PUB4) E3 ubiquitin ligase as being necessary for this process. pub4-6 mutants had defects in stress adaptation and longevity. Thus, we have identified a signal that leads to the targeted removal of ROS-overproducing chloroplasts.

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