Crystal Structure of a CAP-DNA Complex: the DNA Is Bent by 90° | Zendy

Steve C. Schultz | Zendy; George C. Shields | Zendy; Thomas A. Steitz | Zendy

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Crystal Structure of a CAP-DNA Complex: the DNA Is Bent by 90°

Author(s) -

Steve C. Schultz,

George C. Shields,

Thomas A. Steitz

Publication year - 1991

Publication title -

science

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 12.556

H-Index - 1186

eISSN - 1095-9203

pISSN - 0036-8075

DOI - 10.1126/science.1653449

Subject(s) - dna , base pair , crystallography , bent molecular geometry , transcription (linguistics) , crystal structure , activator (genetics) , biology , gene , chemistry , biophysics , genetics , linguistics , philosophy , organic chemistry

The 3 angstrom resolution crystal structure of the Escherichia coli catabolite gene activator protein (CAP) complexed with a 30-base pair DNA sequence shows that the DNA is bent by 90 degrees. This bend results almost entirely from two 40 degrees kinks that occur between TG/CA base pairs at positions 5 and 6 on each side of the dyad axis of the complex. DNA sequence discrimination by CAP derives both from sequence-dependent distortion of the DNA helix and from direct hydrogen-bonding interactions between three protein side chains and the exposed edges of three base pairs in the major groove of the DNA. The structure of this transcription factor--DNA complex provides insights into possible mechanisms of transcription activation.

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