Conformational dynamics of single HIV-1 envelope trimers on the surface of native virions | Zendy

James B. Munro | Zendy; Jason Gorman | Zendy; Xiaochu Ma | Zendy; Zhou Zhou | Zendy; James Arthos | Zendy; Dennis R. Burton | Zendy; Wayne C. Koff | Zendy; Joel R. Courter | Zendy; Amos B. Smith | Zendy; Peter D. Kwong | Zendy; Scott C. Blanchard | Zendy; Walther Mothes | Zendy

AI Assistant Blog Pricing

Home ZAIA Blog

Open Access

Conformational dynamics of single HIV-1 envelope trimers on the surface of native virions

Author(s) -

James B. Munro,

Jason Gorman,

Xiaochu Ma,

Zhou Zhou,

James Arthos,

Dennis R. Burton,

Wayne C. Koff,

Joel R. Courter,

Amos B. Smith,

Peter D. Kwong,

Scott C. Blanchard,

Walther Mothes

Publication year - 2014

Publication title -

science

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 12.556

H-Index - 1186

eISSN - 1095-9203

pISSN - 0036-8075

DOI - 10.1126/science.1254426

Subject(s) - förster resonance energy transfer , neutralization , viral envelope , context (archaeology) , glycoprotein , biophysics , chemistry , immune escape , protein subunit , ligand (biochemistry) , human immunodeficiency virus (hiv) , protein structure , virology , virus , biology , fluorescence , receptor , immune system , biochemistry , gene , physics , genetics , quantum mechanics , paleontology

The HIV-1 envelope (Env) mediates viral entry into host cells. To enable the direct imaging of conformational dynamics within Env, we introduced fluorophores into variable regions of the glycoprotein gp120 subunit and measured single-molecule fluorescence resonance energy transfer within the context of native trimers on the surface of HIV-1 virions. Our observations revealed unliganded HIV-1 Env to be intrinsically dynamic, transitioning between three distinct prefusion conformations, whose relative occupancies were remodeled by receptor CD4 and antibody binding. The distinct properties of neutralization-sensitive and neutralization-resistant HIV-1 isolates support a dynamics-based mechanism of immune evasion and ligand recognition.

The content you want is available to Zendy users.

Already have an account? Click here to sign in.

Having issues? You can contact us here

Accelerating Research