Delineating Antibody Recognition in Polyclonal Sera from Patterns of HIV-1 Isolate Neutralization | Zendy

Ivelin S. Georgiev | Zendy; Nicole A. DoriaRose | Zendy; Tongqing Zhou | Zendy; Young Do Kwon | Zendy; Ryan P. Staupe | Zendy; Stephanie Moquin | Zendy; GwoYu Chuang | Zendy; Mark K. Louder | Zendy; Stephen D. Schmidt | Zendy; Han Altae-Tran | Zendy; Robert T. Bailer | Zendy; Krisha McKee | Zendy; Martha Nason | Zendy; Sijy O’Dell | Zendy; Gilad Ofek | Zendy; Marie Pancera | Zendy; Sanjay Srivatsan | Zendy; Lawrence Shapiro | Zendy; Mark Connors | Zendy; Stephen A. Migueles | Zendy; Lynn Morris | Zendy; Yoshiaki Nishimura | Zendy; Malcolm A. Martin | Zendy; John R. Mascola | Zendy; Peter D. Kwong | Zendy

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Delineating Antibody Recognition in Polyclonal Sera from Patterns of HIV-1 Isolate Neutralization

Author(s) -

Ivelin S. Georgiev,

Nicole A. DoriaRose,

Tongqing Zhou,

Young Do Kwon,

Ryan P. Staupe,

Stephanie Moquin,

GwoYu Chuang,

Mark K. Louder,

Stephen D. Schmidt,

Han Altae-Tran,

Robert T. Bailer,

Krisha McKee,

Martha Nason,

Sijy O’Dell,

Gilad Ofek,

Marie Pancera,

Sanjay Srivatsan,

Lawrence Shapiro,

Mark Connors,

Stephen A. Migueles,

Lynn Morris,

Yoshiaki Nishimura,

Malcolm A. Martin,

John R. Mascola,

Peter D. Kwong

Publication year - 2013

Publication title -

science

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 12.556

H-Index - 1186

eISSN - 1095-9203

pISSN - 0036-8075

DOI - 10.1126/science.1233989

Subject(s) - immunogen , polyclonal antibodies , somatic hypermutation , neutralization , epitope , virology , antibody , biology , germline , monoclonal antibody , immunology , genetics , b cell , gene

Serum characterization and antibody isolation are transforming our understanding of the humoral immune response to viral infection. Here, we show that epitope specificities of HIV-1-neutralizing antibodies in serum can be elucidated from the serum pattern of neutralization against a diverse panel of HIV-1 isolates. We determined "neutralization fingerprints" for 30 neutralizing antibodies on a panel of 34 diverse HIV-1 strains and showed that similarity in neutralization fingerprint correlated with similarity in epitope. We used these fingerprints to delineate specificities of polyclonal sera from 24 HIV-1-infected donors and a chimeric siman-human immunodeficiency virus-infected macaque. Delineated specificities matched published specificities and were further confirmed by antibody isolation for two sera. Patterns of virus-isolate neutralization can thus afford a detailed epitope-specific understanding of neutralizing-antibody responses to viral infection.

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