A Mitochondrial Pyruvate Carrier Required for Pyruvate Uptake in Yeast, Drosophila , and Humans | Zendy

Daniel K. Bricker | Zendy; Eric B. Taylor | Zendy; John C. Schell | Zendy; Thomas Orsak | Zendy; Audrey Boutron | Zendy; Yu-Chan Chen | Zendy; James E. Cox | Zendy; Caleb M. Cardon | Zendy; Jonathan G. Van Vranken | Zendy; Noah Dephoure | Zendy; Claire Redin | Zendy; Sihem Boudina | Zendy; Steven P. Gygi | Zendy; M. Brivet | Zendy; Carl S. Thummel | Zendy; Jared Rutter | Zendy

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A Mitochondrial Pyruvate Carrier Required for Pyruvate Uptake in Yeast, Drosophila , and Humans

Author(s) -

Daniel K. Bricker,

Eric B. Taylor,

John C. Schell,

Thomas Orsak,

Audrey Boutron,

Yu-Chan Chen,

James E. Cox,

Caleb M. Cardon,

Jonathan G. Van Vranken,

Noah Dephoure,

Claire Redin,

Sihem Boudina,

Steven P. Gygi,

M. Brivet,

Carl S. Thummel,

Jared Rutter

Publication year - 2012

Publication title -

science

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 12.556

H-Index - 1186

eISSN - 1095-9203

pISSN - 0036-8075

DOI - 10.1126/science.1218099

Subject(s) - citric acid cycle , pyruvate decarboxylation , pyruvate dehydrogenase complex , pyruvate dehydrogenase kinase , biochemistry , mitochondrion , glycolysis , pyruvate carboxylase , yeast , pkm2 , transporter , biology , pyruvate dehydrogenase phosphatase , microbiology and biotechnology , chemistry , metabolism , pyruvate kinase , gene , enzyme

Pyruvate constitutes a critical branch point in cellular carbon metabolism. We have identified two proteins, Mpc1 and Mpc2, as essential for mitochondrial pyruvate transport in yeast, Drosophila, and humans. Mpc1 and Mpc2 associate to form an ~150-kilodalton complex in the inner mitochondrial membrane. Yeast and Drosophila mutants lacking MPC1 display impaired pyruvate metabolism, with an accumulation of upstream metabolites and a depletion of tricarboxylic acid cycle intermediates. Loss of yeast Mpc1 results in defective mitochondrial pyruvate uptake, and silencing of MPC1 or MPC2 in mammalian cells impairs pyruvate oxidation. A point mutation in MPC1 provides resistance to a known inhibitor of the mitochondrial pyruvate carrier. Human genetic studies of three families with children suffering from lactic acidosis and hyperpyruvatemia revealed a causal locus that mapped to MPC1, changing single amino acids that are conserved throughout eukaryotes. These data demonstrate that Mpc1 and Mpc2 form an essential part of the mitochondrial pyruvate carrier.

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