High-Resolution Protein Structure Determination by Serial Femtosecond Crystallography | Zendy

Sébastien Boutet | Zendy; Lukas Lomb | Zendy; Garth J. Williams | Zendy; Thomas R. M. Barends | Zendy; Andrew Aquila | Zendy; R. Bruce Doak | Zendy; Uwe Weierstall | Zendy; Daniel P. DePonte | Zendy; Jan Steinbrener | Zendy; Robert L. Shoeman | Zendy; M. Messerschmidt | Zendy; Anton Barty | Zendy; Thomas A. White | Zendy; Stephan Kassemeyer | Zendy; Richard A. Kirian | Zendy; M. Seibert | Zendy; P. Montanez | Zendy; C. Kenney | Zendy; Ryan Herbst | Zendy; Philip Hart | Zendy; J. Pines | Zendy; G. Haller | Zendy; Sol M. Grüner | Zendy; Hugh T. Philipp | Zendy; Mark W. Täte | Zendy; Marianne S. Hromalik | Zendy; Lucas J. Koerner | Zendy; Niels van Bakel | Zendy; J. Morse | Zendy; W. Ghonsalves | Zendy; David Arnlund | Zendy; Michael J. Bogan | Zendy; Carl Caleman | Zendy; Raimund Fromme | Zendy; Christina Y. Hampton | Zendy; Mark S. Hunter | Zendy; Linda C. Johansson | Zendy; Gergely Katona | Zendy; Christopher Kupitz | Zendy; Mengning Liang | Zendy; Andrew V. Martin | Zendy; Karol Nass | Zendy; Lars Redecke | Zendy; Francesco Stellato | Zendy; Nicuşor Tı̂mneanu | Zendy; Dingjie Wang | Zendy; Nadia A. Zatsepin | Zendy; Donald W. Schafer | Zendy; James Defever | Zendy; Richard Neutze | Zendy; Petra Fromme | Zendy; John C. H. Spence | Zendy; Henry N. Chapman | Zendy; Ilme Schlichting | Zendy

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High-Resolution Protein Structure Determination by Serial Femtosecond Crystallography

Author(s) -

Sébastien Boutet,

Lukas Lomb,

Garth J. Williams,

Thomas R. M. Barends,

Andrew Aquila,

R. Bruce Doak,

Uwe Weierstall,

Daniel P. DePonte,

Jan Steinbrener,

Robert L. Shoeman,

M. Messerschmidt,

Anton Barty,

Thomas A. White,

Stephan Kassemeyer,

Richard A. Kirian,

M. Seibert,

P. Montanez,

C. Kenney,

Ryan Herbst,

Philip Hart,

J. Pines,

G. Haller,

Sol M. Grüner,

Hugh T. Philipp,

Mark W. Täte,

Marianne S. Hromalik,

Lucas J. Koerner,

Niels van Bakel,

J. Morse,

W. Ghonsalves,

David Arnlund,

Michael J. Bogan,

Carl Caleman,

Raimund Fromme,

Christina Y. Hampton,

Mark S. Hunter,

Linda C. Johansson,

Gergely Katona,

Christopher Kupitz,

Mengning Liang,

Andrew V. Martin,

Karol Nass,

Lars Redecke,

Francesco Stellato,

Nicuşor Tı̂mneanu,

Dingjie Wang,

Nadia A. Zatsepin,

Donald W. Schafer,

James Defever,

Richard Neutze,

Petra Fromme,

John C. H. Spence,

Henry N. Chapman,

Ilme Schlichting

Publication year - 2012

Publication title -

science

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 12.556

H-Index - 1186

eISSN - 1095-9203

pISSN - 0036-8075

DOI - 10.1126/science.1217737

Subject(s) - femtosecond , protein crystallization , crystallography , resolution (logic) , micrometer , diffraction , x ray crystallography , synchrotron , synchrotron radiation , laser , materials science , crystallization , phaser , macromolecule , lysozyme , high resolution , molecule , chemistry , optics , physics , computer science , biochemistry , remote sensing , organic chemistry , artificial intelligence , geology

Structure determination of proteins and other macromolecules has historically required the growth of high-quality crystals sufficiently large to diffract x-rays efficiently while withstanding radiation damage. We applied serial femtosecond crystallography (SFX) using an x-ray free-electron laser (XFEL) to obtain high-resolution structural information from microcrystals (less than 1 micrometer by 1 micrometer by 3 micrometers) of the well-characterized model protein lysozyme. The agreement with synchrotron data demonstrates the immediate relevance of SFX for analyzing the structure of the large group of difficult-to-crystallize molecules.

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