Structural Basis for Ribosome Recruitment and Manipulation by a Viral IRES RNA | Zendy

Jennifer S. Pfingsten | Zendy; David A. Costantino | Zendy; Jeffrey S. Kieft | Zendy

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Structural Basis for Ribosome Recruitment and Manipulation by a Viral IRES RNA

Author(s) -

Jennifer S. Pfingsten,

David A. Costantino,

Jeffrey S. Kieft

Publication year - 2006

Publication title -

science

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 12.556

H-Index - 1186

eISSN - 1095-9203

pISSN - 0036-8075

DOI - 10.1126/science.1133281

Subject(s) - internal ribosome entry site , ribosome , translation (biology) , eukaryotic ribosome , rna , 5.8s ribosomal rna , microbiology and biotechnology , eukaryotic translation , computational biology , biology , ribosome profiling , ribosomal rna , genetics , messenger rna , gene

Canonical cap-dependent translation initiation requires a large number of protein factors that act in a stepwise assembly process. In contrast, internal ribosomal entry sites (IRESs) are cis-acting RNAs that in some cases completely supplant these factors by recruiting and activating the ribosome using a single structured RNA. Here we present the crystal structures of the ribosome-binding domain from a Dicistroviridae intergenic region IRES at 3.1 angstrom resolution, providing a view of the prefolded architecture of an all-RNA translation initiation apparatus. Docking of the structure into cryo-electron microscopy reconstructions of an IRES-ribosome complex suggests a model for ribosome manipulation by a dynamic IRES RNA.

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