Phase Separation of Lipid Membranes Analyzed with High-Resolution Secondary Ion Mass Spectrometry | Zendy

Mary L. Kraft | Zendy; Peter Weber | Zendy; Marjorie L. Longo | Zendy; I. D. Hutcheon | Zendy; Steven G. Boxer | Zendy

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Phase Separation of Lipid Membranes Analyzed with High-Resolution Secondary Ion Mass Spectrometry

Author(s) -

Mary L. Kraft,

Peter Weber,

Marjorie L. Longo,

I. D. Hutcheon,

Steven G. Boxer

Publication year - 2006

Publication title -

science

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 12.556

H-Index - 1186

eISSN - 1095-9203

pISSN - 0036-8075

DOI - 10.1126/science.1130279

Subject(s) - mass spectrometry , resolution (logic) , membrane , chemistry , chromatography , high resolution , ion , secondary ion mass spectrometry , analytical chemistry (journal) , phase (matter) , biochemistry , computer science , geology , organic chemistry , remote sensing , artificial intelligence

Lateral variations in membrane composition are postulated to play a central role in many cellular events, but it has been difficult to probe membrane composition and organization on length scales of tens to hundreds of nanometers. We present a high-resolution imaging secondary ion mass spectrometry technique to reveal the lipid distribution within a phase-separated membrane with a lateral resolution of approximately 100 nanometers. Quantitative information about the chemical composition within small lipid domains was obtained with the use of isotopic labels to identify each molecular species. Composition variations were detected within some domains.

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