Open AccessComplete Replication of Hepatitis C Virus in Cell Culture
Author(s) -
Brett D. Lindenbach,
Matthew J. Evans,
Andrew J. Syder,
Benno Wölk,
Timothy L. Tellinghuisen,
Christopher C. Liu,
Toshiaki Maruyama,
Richard O. Hynes,
Dennis R. Burton,
Jane A. McKeating,
Charles M. Rice
Publication year - 2005
Publication title -
science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 12.556
H-Index - 1186
eISSN - 1095-9203
pISSN - 0036-8075
DOI - 10.1126/science.1114016
Subject(s) - virology , hepatitis c virus , biology , virus , viral replication , monoclonal antibody , in vitro , interferon , cell culture , hepacivirus , ns2 3 protease , cd81 , antibody , immunology , genetics
Many aspects of the hepatitis C virus (HCV) life cycle have not been reproduced in cell culture, which has slowed research progress on this important human pathogen. Here, we describe a full-length HCV genome that replicates and produces virus particles that are infectious in cell culture (HCVcc). Replication of HCVcc was robust, producing nearly 10(5) infectious units per milliliter within 48 hours. Virus particles were filterable and neutralized with a monoclonal antibody against the viral glycoprotein E2. Viral entry was dependent on cellular expression of a putative HCV receptor, CD81. HCVcc replication was inhibited by interferon-alpha and by several HCV-specific antiviral compounds, suggesting that this in vitro system will aid in the search for improved antivirals.
Accelerating Research
Robert Robinson Avenue,
Oxford Science Park, Oxford
OX4 4GP, United Kingdom
Address
John Eccles HouseRobert Robinson Avenue,
Oxford Science Park, Oxford
OX4 4GP, United Kingdom