Endosomal Proteolysis of the Ebola Virus Glycoprotein Is Necessary for Infection | Zendy

Kartik Chandran | Zendy; Nancy J. Sullivan | Zendy; Ute Felbor | Zendy; Sean P. J. Whelan | Zendy; James M. Cunningham | Zendy

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Endosomal Proteolysis of the Ebola Virus Glycoprotein Is Necessary for Infection

Author(s) -

Kartik Chandran,

Nancy J. Sullivan,

Ute Felbor,

Sean P. J. Whelan,

James M. Cunningham

Publication year - 2005

Publication title -

science

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 12.556

H-Index - 1186

eISSN - 1095-9203

pISSN - 0036-8075

DOI - 10.1126/science.1110656

Subject(s) - ebola virus , filoviridae , virology , cathepsin l , vero cell , biology , cathepsin b , proteases , endosome , vesicular stomatitis virus , ebolavirus , mononegavirales , protease , glycoprotein , virus , enzyme , microbiology and biotechnology , cell , viral disease , paramyxoviridae , biochemistry

Ebola virus (EboV) causes rapidly fatal hemorrhagic fever in humans and there is currently no effective treatment. We found that the infection of African green monkey kidney (Vero) cells by vesicular stomatitis viruses bearing the EboV glycoprotein (GP) requires the activity of endosomal cysteine proteases. Using selective protease inhibitors and protease-deficient cell lines, we identified an essential role for cathepsin B (CatB) and an accessory role for cathepsin L (CatL) in EboV GP-dependent entry. Biochemical studies demonstrate that CatB and CatL mediate entry by carrying out proteolysis of the EboV GP subunit GP1 and support a multistep mechanism that explains the relative contributions of these enzymes to infection. CatB and CatB/CatL inhibitors diminish the multiplication of infectious EboV-Zaire in cultured cells and may merit investigation as anti-EboV drugs.

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